Department of Mycology (2005 - Present)
Mycology
, Tarbiat Modares University,
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name: shahla surname: roudbar mohammadi Married occupation date :from 2005- peresent filed: medical mycology Bsc: Biology Mcs: Medical mycology Phd: Medical mycology -Honor: first rank on Phd student first rank on Msc educated beter resercher 2013 -Reserch: Biofilm nano particles and evaluation of its anti fungal effects molecular diagnosis of fungi -BOOKS fungal pathogenesis Biofilm Reserch projects: evaluation of cold plasma on candida albicans and asperjilus evaluation of Immunomadulatory of candida ceell wall fungal infections on syssticfibrosis
Candida albicans (C. albicans) belongs to the opportunistic fungal pathogens, which cause a wide spectrum of infections in immune-compromised patients. Graphene oxide (GO), a biocompatibility agent, has been reported to exhibit effective antimicrobial activity. In the present study, a graphene oxide/fluconazole (GO/Flu) compound was synthesized and characterized using Fourier transform infrared spectroscopy (FTIR) and Raman spectroscopy. The antifungal activity of GO/Flu was examined against fluconazole-resistant C. albicans (ATCC 10231) compared to GO and Flu using the broth microdilution method, according to CLSI protocol. DNA fragmentation was assessed through the antifungal mechanism of GO/Flu. The release of Fluin PBS medium was measur
Background: The importance of Candida albicans as the most common cause of fungal infections in humans is undeniable. Genotyping methods have been developed as useful tools to differentiate between fungal strains isolated from various infections. Several molecular typing methods have been described for C. albicans, and fragment length analysis of microsatellites called microsatellite fragment length polymorphism (MLP) is one of the most accurate genotyping methods.Objectives: The present study aimed at evaluating the genetic diversity and genetic relationships among C. albicans isolates recovered from HIV-positive patients with oral candidiasis in Iran using MLP.Methods: We analyzed 30 isolates of C. albicans obtained from HIV-positive pati
The aim of this study was to evaluate the ability of heat-killed baker's yeast (HKBY), the cell wall of baker's yeast (CWBY), and cell wall (1→3)-β-d-glucan of baker's yeast (BGBY) to bind aflatoxin B1 (AFB1) in phosphate-buffered saline (PBS) spiked with 0.5 μg/mL AFB1. Baker's yeast (Saccharomyces cerevisiae) was heat killed by autoclaving at 121?C for 10 min. The cell wall was physically extracted, and (1→3)-β-d-glucan was extracted by a modified method. The concentration of AFB1 was determined by high-performance liquid chromatography after exposure to binders for three contact times, 30 min, 5 h, and 24 h, at room temperature. AFB1 binding by HKBY, CWBY, and BGBY was 6.30 to 46.34%. The lowest binding capacity was found for
Background: Drug resistance in Candida species, has emerged as a major problem in the public health system worldwide. Application of nanoparticles is proposed as a novel and potential agent for reduction of drug resistance burdens.Objectives: The current study was conducted to evaluate the effects of zinc oxide nanoparticles (ZnO NPs containing chitosan and linoleic acid) on hyphae cell wall proteins (Hwp1) gene expression, a crucial gene in pathogenicity of Candida albicans, and cytotoxicity on human hepatocyte carcinoma (HepG2) cells as well as the production of Reactive Oxygen Species (ROS).Methods: The effects of novel ZnO NPs on expression of Hwp1 gene of C. albicans was analyzed using quantitative real-time polymerase chain reaction (
RNA processing is essential factor for synthesis of functional and structural proteins in eukaryote cells. In eukaryote organisms it will be initiated with transcription of DNA in nucleolus and terminated to mRNA translation in cytoplasm, finally mRNA degraded. Protein synthesis followed as different steps, includes 5'capping, poly adenylating, processing and transferring from nucleolus to cytoplasm that all processing control and regulate MicroRNA are small molecules that could be inhibit the translation or degrade the mRNA. Due to the main biological role of RNA in regulation of protein synthesis, it is obvious that fungal pathogenesis is highly related to RNA. Interfrence RNAs (iRNA) are small double strand RNA in fungi that produced by
Background: Although Malassezia genusare part of the skin normal flora, under certain conditions, they become pathogenic. Catheter-related fungemia, caused by Malassezia, which is associated with biofilm formation, is considered a nosocomial infection.Objectives: The aim of this study was to evaluate the ability of Malassezia globosa and Malassezia restricta in biofilm formation.Methods: Biofilm formation was carried out using catheter segments in 12-well plates. Results were measured using 3-(4, 5-dimethylthiazol-2-yl)-2, 5-diphenyl-tetrazolium bromide (MTT) colorimetric assay in 96-well plates. The data was analyzed using univariate Analysis of Variance (ANOVA) or Repeated Measures ANOVA. P values of≤ 0.05 were considered statistically
The potential of natural substances with immunotherapeutic properties has long been studied. β-glucans, a cell wall component of certain bacteria and fungi, potentiate the immune system against microbes and toxic substances. Moreover, β-glucans are known to exhibit direct anticancer?effects and can suppress cancer proliferation through immunomodulatory pathways. Mortality of lung cancer has been alarmingly increasingly worldwide; therefore,?treatment of lung cancer is an urgent necessity. Numerous researchers are now dedicated to using β-glucans as a therapy for lung cancer. In the present attempt, we have reviewed the studies addressing therapeutic effects of β-glucans in primary and metastatic lung cancer published in t
Context: Curcumin, a naturally occurring polyphenol, has been extensively studied for its broad-spectrum anticancer effects. The potential benefits are, however, limited due to its poor water solubility and rapid degradation which result in low bioavailability on administration.Objectives: This study encapsulates curcumin in nanoliposomes including an integrin-homing peptide combined with a C end R neuropilin-1 targeting motif for targeted delivery and receptor-mediated internalization, respectively.Materials and methods: The linear GHHNGR (Glycine–Histidine–Histidine–Asparagine–Glycine–Arginine) was synthesized through F-moc chemistry on 2-chlorotrityl chloride resin and conjugated to oleic acid. The lipoyl-peptide units were the
Over the last decade, communication industries have witnessed a tremendous expansion, while, the biological effects of electromagnetic waves have not been fully elucidated. Current study aimed at evaluating the mutagenic effect of long-term exposure to 900-MHz radiation on alpha-Int1 gene sequences of Candida albicans. A standard 900?MHz radiation generator was used for radiation. 10?ml volumes from a stock suspension of C. albicans were transferred into 10 polystyrene tubes. Five tubes were exposed at 4??C to a fixed magnitude of radiation with different time periods of 10, 70, 210, 350 and 490?h. The other 5 tubes were kept far enough from radiation. The samples underwent genomic DNA extraction. PCR amplification of alpha-Int1 gene sequen
Background: This study was performed to determine antifungal activity of silver nanoparticles (nano-Ag) compared to voriconazole on clinical and standard strains of Aspergillus fumigatus.Materials and Methods: Inhibitory potency of nano-Ag was determined using microtiter broth dilution method. Susceptibility tests were performed against A. fumigatus isolated from BAL (bronchoalveolar lavage) of patients who suffered from respiratory problems and compared with the strain (ATCC: 204305) by broth dilution antifungal susceptibility test of filamentous fungi approved by the Clinical and Laboratory Standards Institute M38-A. In addition, cytotoxicity effect of silver nanoparticles was studied on epithelial cell line by MTT assay. Results: From 60
Malassezia genus in dandruff and seborrheic dermatitis which together have affected more than 50% of humans, have increasing proliferation in scalp. Proliferation of yeasts lead to scalp-flaking and create physical and mental disorder in people. The conventional culture-based methods for the isolation and identification of the Malassezia species in relative disease are labour intensive and time-consuming. We aimed to conduct a molecular analysis with direct DNA extraction from scalp in such patients at least time without complexity. In this cross sectional study, samples were taken from 65 patients with dandruff and seborrheic dermatitis. DNA extraction was performed directly from uncultured scalp by hexadecyltrimethylammonium bromide (CTAB
Aim and Background: Vulvovaginal candidiasis is one of the most common female genital tract infections that is caused by overgrowth Candida species, especially Candida albicans and sometimes becomes chronic and recurrent form. Candida glabrata is the second or third leading cause of candidiasis after C. albicans. Following the widespread and increased use of immunosuppressive therapy along with broad-spectrum antimycotic therapy, the frequency of mucosal and systemic infections caused by C. glabrata has increased significantly. Fluconazole is one of the most common drugs used for treatment of this type of candidiasis but resistant is also seen. The first step in infection is adherence to epithelial cells. This study was designed to evaluate
Mesoporous silica nanoparticles (MCM-41) have an ability to increase the solubility of poorly soluble drugs. In this study, MCM-41 was synthesized. Afterward, Itraconazole (ITZ) was loaded into MCM-41 and then was wrapped by chitosan. The synthesized nanoparticles were characterized. Controlled release of ITZ from the MSNITZ and MSN-ITZ-CHI was evaluated in hydrochloride acid buffer (pH: 1.2) and phosphate buffer saline (pH: 7.4). Antifungal and cytotoxic activities of MSN-ITZ and MSN-ITZ-CHI were evaluated. Amount of loaded ITZ into MCM-41 was determined 85%. The properties of MCM-41 and loading procedure revealed high performance of the drug loading and release studies. The release profiles of MSN-ITZ were 60% in HCl medium and 40% in PBS
To date, registered users of mobile phone communication network exceeded from total numbers of the world population, while a little knowledge of the biological effects of, 900-1800 MHz microwave radiation, originating from the handsets or the base transceiver stations, have been released. The current study was designed for evaluation of 900-MHz radiation effects on Candida albicans proliferation, adherence and alpha-Int1 gene expression.
BACKGROUND AND AIM: CANDIDASPP. PRODUCE BIOFILMS ON SYNTHETIC MATERIALS SUCH AS CATHETER, WHICH FACILITATES ADHESION OF THE ORGANISMS TO DEVICES AND RENDERS THEM RELATIVELY REFRACTORY TO MEDICAL THERAPY. THE PURPOSE OF THIS STUDY IS; THE USE OF ZINC OXIDE NANOPARTICLES IN THE MANUFACTURE CATHETER AND EVALUATION OF ITS INHIBITORY EFFECT IN PREVENTING OF THE BIOFILM FORMATION...
Patients with diabetes demonstrated to be more prone to developing infections than others. Diabetic foot ulcer is a major health problem in these patients. The purpose of the study was to investigate the incidence of fungal and bacterial pathogens in diabetic foot infections. A total of 57 diabetic patients with non-healing diabetic foot infections who were referred to diabetic clinic during 2011 to 2012 were included for the study. Samples were obtained from the depth of the wound by the use of a sterile swab. Direct fresh smear, fungal and bacterial culture were performed for each patient. Fungal contaminations were confirmed by direct microscopy and/or culture and molecular method. In this study, 9 patients (15.7%) did not show any bacte
Background: In this study, for the first time, the mutagenic impact of long-term exposure to 900-MHz radiation on Can-dida albicans alpha-Int1 gene sequences were evaluated.Methods: A standard EMF generator with output ranging from 860 to 940 MHz was used for radiation. 10 ml volumes from a stock suspension of Candida albicans (2x10^ 6 cell/ml, in log phase of growth, prepared in PBS) were transferred into ten 15 ml volume polystyrene tubes. 5 tubes were incu-bated at 4 C and exposed (distance= 0~ 15 cm) to fixed mag-nitude of radiation with different time periods of 10, 70, 210,350 and 490 hours. The other 5 tubes were kept at the same conditions but far enough from radiation generator. Exposed and unexposed samples went under genomic DNA
Abstract Introduction and Objectives: Pathogenic yeasts from the genus Candida can be the reason of serious infections in human especially, in immune compromised patients and are now known as major agents of nosocomial infections. Many such manifestation of infections associate with the formation of Candida biofilms. This structures are extremely resistant to chemical drugs and are cause of many problems in medicine. The aim of this study was to use the extract of Myrtus communis to inhabit biofilm formation by Candida tropicalis (ATCC0750). Materials and Methods: In this study, the extract of M. communis was prepared and then the concentration of extract was measured. The minimum inhibitory concentration (MICs) and minimum fungicidal conce
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